168.

Fritz JC, Pla GW, Boehne JW. Influence of chelating agents on utilization of calcium, iron and manganese by the chick. Poultry Sci. 1971; 50(5): 1444-1450. (CA75) [Chick diets, designed to be borderline in Ca, Fe, and Mn, were fed with and without addn. of common chelating agents. Toe ash was studied as a criterion of Ca utilization, Hb and hematocrit as a measure of Fe utilization, and perosis score as a measure of Mn utilization. The form of EDTA was found to be important. High levels of the free acid form were most detrimental to utilization of Fe and Mn, and adverse effects were noted when 100 ppm was included in the diet. About 800 ppm of di-Na EDTA or Ca di-Na EDTA was required to significantly depress Hb and hematocrit. Inclusion of 800 ppm EDTA depressed growth and Hb, and increased incidence and severity of perosis, to about the same extent as did 1600 ppm of di-Na EDTA or Ca di-Na EDTA. Fe furnished by the dihydrogen ferrous salt of EDTA (FeH2EDTA) was available to the chick. Other materials studied, including nitrilotriacetic acid, oxalic acid, tetracycline, hetacillin, Ca phytate, and kaolin had lesser effects on utilization of Fe and Mn. None of the materials tested interfered with utilization of Ca.]

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