82.

Pagnotto LD, Elkins HB, Bayka I. Oral administration of edathamil calcium disodium (calcium disodium versenate). AMA Archives of Ind. Health. 1958; 17:29-33 (Jan.). (P1943) [Two subjects, A and B, treated themselves orally (Jan.) with a total of 10 g CaNa2EDTA in tablet form over a period of 4 days. Urine samples were collected daily at ~5 PM. Pb excretion in the urine increased during the 1st days of treatment but fell off when EDTA was discontinued. In subject A, a maximum Pb excretion of 0.47 mg/l urine was reached; in subject B, it was elevated but less high than in subject A. About 6 mo later, subject A and a new subject C repeated the experiment, ingesting a daily dose of 1.4 g CaNa2EDTA (half of the amount of experiment 1) until the urinary Pb excretion reached pretreatment levels. This 2nd course did not stimulate Pb excretion in subject A to the previous level while Pb values of subject C paralleled the earlier results in subject A.
A method for the determination of free EDTA in the urine by adding excess Pb was worked out and it was found that each molecule of EDTA prevented the precipitation of ~2 Pb atoms. Thus, the EDTA in experiment 1 complexed in the urine of subject A and B 26 and 19 mg Pb, respectively, while in experiment 2 the complexed amount of Pb was 31 and 40 mg, respectively, for subject A and C (after totals of 10 and 11 g EDTA), Based on the estimate that each molecule EDTA precipitates 2 atoms of Pb, the absorption of EDTA was <0.3% although there was enough free EDTA in the urine to complex up to 7 mg Pb/l urine. After treatment was stopped, EDTA was fairly rapidly eliminated, and after 3 days little or none was found in the urine. The coprecipitation method for the determination of Pb in the urine of patients treated with EDTA is described. It consists of adding excess dichromate to the urine, reducing with arsenous acid and precipitating the freed Pb by Ca oxalate.

Gordon Research Institute / Reference Material

Gordon Research Institute    Dr. Garry F. Gordon MD DO MD(H)
600 N Beeline Hwy, Suite B, Payson, AZ 85541
Phone: 928-472-4263    Fax: 928-474-3819
Email Dr.Gordon